(A) Testing and inspection done at a facility for issuance of a fish health inspection report shall be done in the following manner:
(1) All lots at a facility shall be inspected and tested using either a polymerase chain reaction test, cell culture test, or another method approved by the department.
(2) Collection of samples from each lot shall be based upon an assumed prevalence level of five per cent and a ninety-five per cent confidence of detecting at least one infected fish in the lot for causative agents of the diseases listed in paragraphs (A)(1)(a) to (A)(1)(g) of rule 901:1-17-13 of the Administrative Code.
If samples cannot be collected for diagnostic testing from all lots of fish at a facility in one day; all lots not sampled shall have samples collected from them within thirty days of the date the first sample was collected at the facility.
(3) Inspection and diagnostic testing for whirling disease shall be done using one sample of sixty fish. The sample shall be made up of fish at an age when whirling disease is most likely to be detected, and from species most susceptible to the disease.
(B) If all lots not sampled in one day at a facility are not sampled within thirty days of the date the first sample was collected; all testing and inspection at the facility shall be done over.