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This website publishes administrative rules on their effective dates, as designated by the adopting state agencies, colleges, and universities.

Rule 3701-53-01 | Techniques or methods.


(A) Tests to determine the concentration of alcohol may be applied to blood, breath, urine, or other bodily substances. Results shall be expressed as equivalent to:

(1) Grams by weight of alcohol per one hundred milliliters of whole blood, blood serum or plasma (grams per cent by weight);

(2) Grams by weight of alcohol per two hundred ten liters of deep lung breath;

(3) Grams by weight of alcohol per one hundred milliliters of urine (grams per cent by weight).


(4) Nanograms by weight of a controlled substances or a metabolite or a controlled substance per milliliter of blood, urine, or other bodily substance.

The results of the tests shall be retained for not less than three years.

(B) At least one copy of the written procedure manual required by paragraph (D) of rule 3701-53-06 of the Administrative Code for performing blood, urine, or other bodily substance tests shall be on file in the area where the analytical tests are performed.

Last updated November 22, 2022 at 3:47 PM

Supplemental Information

Authorized By: 3701.143
Amplifies: 15.47.11, 15.47.111, 4511.19, 4511.191, 4511.192
Five Year Review Date: 5/9/2020
Prior Effective Dates: 3/1/1968, 2/1/1976, 3/15/1983 (Emer.), 6/13/1983, 1/1/1987, 7/7/1997, 9/30/2002