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This website publishes administrative rules on their effective dates, as designated by the adopting state agencies, colleges, and universities.

Rule 3701-53-05 | Collection and handling of blood and urine specimens.


(A) All samples shall be collected in accordance with section 4511.19, or section 1547.11 of the Revised Code, as applicable.

(B) When collecting a blood sample, an aqueous solution of a non-volatile antiseptic shall be used on the skin. No alcohols shall be used as a skin antiseptic.

(C) Blood shall be drawn with a sterile dry needle into a vacuum container with a solid anticoagulant, or according to the laboratory protocol as written in the laboratory procedure manual based on the type of specimen being tested.

(D) The collection of a urine specimen must be witnessed to assure that the sample can be authenticated. Urine shall be deposited into a clean glass or plastic screw top container which shall be capped, or collected according to the laboratory protocol as written in the laboratory procedure manual

(E) Blood and urine containers shall be sealed in a manner such that tampering can be detected and have a label which contains at least the following information:

(1) Name of suspect;

(2) Date and time of collection;

(3) Name or initials of person collecting the sample; and

(4) Name or initials of person sealing the sample.

(F) While not in transit or under examination, all blood and urine specimens shall be refrigerated.

Last updated November 4, 2022 at 4:56 PM

Supplemental Information

Authorized By: 3701.143
Amplifies: 15.47.11, 15.47.111, 4511.19, 4511.191, 4511.192
Five Year Review Date: 5/9/2020
Prior Effective Dates: 3/1/1968, 3/15/1983 (Emer.), 6/13/1983, 1/1/1987, 5/5/1990, 9/14/1994 (Emer.), 12/12/1994, 1/4/1996 (Emer.), 3/19/1996 (Emer.), 6/13/1996, 7/7/1997, 9/30/2002